E03C0851 Mouse CXC Chemokine Ligand 16 ELISA kit
The Mouse CXC Chemokine Ligand 16 ELISA kit can be used to identify samples from the mouse species. CXC Chemokine Ligand 16 can also be called CXCLG16, SR-PSOX, SRPSOX, CXCL16.
Specifications of Mouse CXC Chemokine Ligand 16 ELISA kit
Product Information | |
Cat. No. | E03C0851 |
Product Name | Mouse CXC Chemokine Ligand 16 ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000pg/ml |
Sensitivity | 1.0pg/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CXCL16 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CXCL16. Standards or samples are then added to the microtiter plate wells and CXCL16 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CXCL16 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CXCL16 are added to each well to “sandwich” the CXCL16 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CXCL16 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CXCL16 concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse CXC Chemokine Ligand 16 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CXCL16 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse CXC Chemokine Ligand 16 ELISA kit
Citations of Mouse CXC Chemokine Ligand 16 ELISA kit
E03C0851 has been referenced in the below publications：
The dynamic changes of CXCL16/ox-LDL and the effects of simvastatin in mice with adriamycin-induced nephropathy.
