Mouse Cyclic AMP Response Element Binding Protein ELISA kit (E03C0552)

BlueGene Biotech Mouse Cyclic AMP Response Element Binding Protein ELISA kit (E03C0552)

Epigenetics and Nuclear Signaling

Immunology

E03C0552 Mouse Cyclic AMP Response Element Binding Protein ELISA kit

The Mouse Cyclic AMP Response Element Binding Protein ELISA kit can be used to identify samples from the mouse species. Cyclic AMP Response Element Binding Protein can also be called CREB1, CAMP Responsive Element Binding Protein 1, CREB．

Products

Specifications of Mouse Cyclic AMP Response Element Binding Protein ELISA kit

Product Information
Cat. No.	E03C0552
Product Name	Mouse Cyclic AMP Response Element Binding Protein ELISA kit
Species	Mouse
Product Size	48 Tests / 96 Tests
Concentration	0.5-10ng/ml
Sensitivity	0.1ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/ml	1 vial
STANDARD B (0.5mL)	0.5 ng/ml	1 vial
STANDARD C (0.5mL)	1.0 ng/ml	1 vial
STANDARD D (0.5mL)	2.5 ng/ml	1 vial
STANDARD E (0.5mL)	5.0ng/ml	1 vial
STANDARD F (0.5mL)	10 ng/ml	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

CREB ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CREB antibody and a CREB-HRP conjugate. The assay sample and buffer are incubated together with CREB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CREB concentration since CREB from samples and CREB-HRP conjugate compete for the anti-CREB antibody binding site. Since the number of sites is limited, as more sites are occupied by CREB from the sample, fewer sites are left to bind CREB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CREB concentration in each sample is interpolated from this standard curve.

Quality Control on Mouse Cyclic AMP Response Element Binding Protein ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between CREB and analogues was observed.