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BlueGene Biotech Mouse Granulocyte Colony Stimulating Factor ELISA kit (E03G0112)

E03G0112 Mouse Granulocyte Colony Stimulating Factor ELISA kit

The Mouse Granulocyte Colony Stimulating Factor ELISA kit can be used to identify samples from the mouse species. Granulocyte Colony Stimulating Factor  can also be called  G-CSF, GCSF, CSF3, C17orf33, CSF3OS, colony stimulating factor 3.

Products

Specifications of Mouse Granulocyte Colony Stimulating Factor ELISA kit

Product Information

Cat. No.

E03G0112

Product Name

Mouse Granulocyte Colony Stimulating Factor ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

50-1000pg/ml

Sensitivity

1.0pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/ml1 vial

STANDARD B (0.5mL)

50 pg/ml

1 vial

STANDARD C (0.5mL)

100 pg/ml

1 vial

STANDARD D (0.5mL)

250 pg/ml

1 vial

STANDARD E (0.5mL)

500 pg/ml

1 vial

STANDARD F (0.5mL)

1000 pg/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

GCSF ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GCSF antibody and an GCSF-HRP conjugate. The assay sample and buffer are incubated together with GCSF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GCSF concentration since GCSF from samples and GCSF-HRP conjugate compete for the anti-GCSF antibody binding site. Since the number of sites is limited, as more sites are occupied by GCSF from the sample, fewer sites are left to bind GCSF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GCSF concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Granulocyte Colony Stimulating Factor ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between GCSF and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Granulocyte Colony Stimulating Factor ELISA kit

Summary of the Assay Procedure for Mouse Granulocyte Colony Stimulating Factor ELISA kit

Citations of Mouse Granulocyte Colony Stimulating Factor ELISA kit

E03G0112 has been referenced in the below publications:

EXPERIMENTAL STUDY OF CAFFEIC ACID COMBINED HYDROGEN-RICH NORMAL SALINE TO TREAT ACUTE RADIATION DISEASE OF BONE MARROW.

Experimental study of caffeicacid combined hydrogen-rich normal saline totreat acute radiation disease of bone marrow.

Experimental study of caffeicacid combined hydrogen-rich normal saline totreat acute radiation disease of bone marrow.


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