E03H0268 Mouse Helicobacter Pylori IgG ELISA kit
The Mouse Helicobacter Pylori IgG ELISA kit can be used to identify samples from the mouse species. Helicobacter Pylori IgG can also be called Hp IgG, Helicobacter pylori IgG.
Specifications of Mouse Helicobacter Pylori IgG ELISA kit
Product Information | |
Cat. No. | E03H0268 |
Product Name | Mouse Helicobacter Pylori IgG ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500ng/mL |
Sensitivity | 1.0ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 25 ng/ml | 1 vial |
STANDARD C (0.5mL) | 50 ng/ml | 1 vial |
STANDARD D (0.5mL) | 100 ng/ml | 1 vial |
STANDARD E (0.5mL) | 250 ng/ml | 1 vial |
STANDARD F (0.5mL) | 500 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Hp IgG ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-Hp IgG antibody and an Hp IgG-HRP conjugate. The assay sample and buffer are incubated together with Hp IgG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Hp IgG concentration since Hp IgG from samples and Hp IgG-HRP conjugate compete for the anti-Hp IgG antibody binding site. Since the number of sites is limited, as more sites are occupied by Hp IgG from the sample, fewer sites are left to bind Hp IgG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Hp IgG concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Helicobacter Pylori IgG ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Hp IgG and analogues was observed. | |
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Summary of the Assay Procedure for Mouse Helicobacter Pylori IgG ELISA kit
Citations of Mouse Helicobacter Pylori IgG ELISA kit
E03H0268 has been referenced in the below publications：
新生BALA/C小鼠口服灭活幽门螺杆菌对特异性抗体的影响作用。
