E03H0013 Mouse Histamine ELISA kit
The Mouse Histamine ELISA kit can be used to identify samples from the mouse species. Histamine can also be called Histamine, Hist, or HIS.
Specifications of Mouse Histamine ELISA kit
Product Information | |
Cat. No. | E03H0013 |
Product Name | Mouse Histamine ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100ng/ml |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
HIS ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-HIS antibody and an HIS-HRP conjugate. The assay sample and buffer are incubated together with HIS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HIS concentration since HIS from samples and HIS-HRP conjugate compete for the anti-HIS antibody binding site. Since the number of sites is limited, as more sites are occupied by HIS from the sample, fewer sites are left to bind HIS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HIS concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Histamine ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between HIS and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Histamine ELISA kit
Citations of Mouse Histamine ELISA kit
E03H0013 has been referenced in the below publications：
Mechanistic study of epidermal growth factor receptor inhibitor-related pruritus.
Ameliorative effect of selective NLRP3 inflammasome inhibitor MCC950 in an ovalbumin-induced allergic rhinitis murine model.
Therapeutic effects of SKF-96365 on murine allergic rhinitis induced by OVA.
