E03I0063 Mouse Immunoglobulin G1 ELISA kit
The Mouse Immunoglobulin G1 ELISA kit can be used to identify samples from the mouse species. Immunoglobulin G1 can also be called IgG1.
Specifications of Mouse Immunoglobulin G1 ELISA kit
Product Information | |
Cat. No. | E03I0063 |
Product Name | Mouse Immunoglobulin G1 ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10mg/ml |
Sensitivity | 0.1mg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 mg/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 mg/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 mg/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 mg/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 mg/mL | 1 vial |
STANDARD F (0.5mL) | 10 mg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IgG1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IgG1 antibody and an IgG1-HRP conjugate. The assay sample and buffer are incubated together with IgG1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IgG1 concentration since IgG1 from samples and IgG1-HRP conjugate compete for the anti-IgG1 antibody binding site. Since the number of sites is limited, as more sites are occupied by IgG1 from the sample, fewer sites are left to bind IgG1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IgG1 concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Immunoglobulin G1 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IgG1 and analogues was observed. | |
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Summary of the Assay Procedure for Mouse Immunoglobulin G1 ELISA kit
Citations of Mouse Immunoglobulin G1 ELISA kit
E03I0063 has been referenced in the below publications：
TRPA1 mediated aggravation of allergic contact dermatitis induced by DINP and regulated by NF-κB activation.
