Mouse Interferon Alpha ELISA kit (E03I0343) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Mouse Interferon Alpha ELISA kit (E03I0343)

E03I0343 Mouse Interferon Alpha ELISA kit

The Mouse Interferon Alpha ELISA kit can be used to identify samples from the mouse species. Interferon Alpha can also be called IFNA1, IFL, LeIF D, IFN, IFN-Alpha, IFNA13, IFN-A, IFNAP22, IFN-Alpha 1b, Interferon Alpha 1b, Interferon, Leukocytic, IFN, Leukocyte, Interferon alpha-D, IFNα.

Products

Specifications of Mouse Interferon Alpha ELISA kit

Product Information
Cat. No.	E03I0343
Product Name	Mouse Interferon Alpha ELISA kit
Species	Mouse
Product Size	48 Tests / 96 Tests
Concentration	50-1000pg/ml
Sensitivity	1.0pg/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/ml	1 vial
STANDARD B (0.5mL)	50 pg/ml	1 vial
STANDARD C (0.5mL)	100 pg/ml	1 vial
STANDARD D (0.5mL)	250 pg/ml	1 vial
STANDARD E (0.5mL)	500 pg/ml	1 vial
STANDARD F (0.5mL)	1000 pg/ml	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

IFNα ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IFNα antibody and an IFNα-HRP conjugate. The assay sample and buffer are incubated together with IFNα-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IFNα concentration since IFNα from samples and IFNα-HRP conjugate compete for the anti-IFNα antibody binding site. Since the number of sites is limited, as more sites are occupied by IFNα from the sample, fewer sites are left to bind IFNα-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFNα concentration in each sample is interpolated from this standard curve.

Quality Control on Mouse Interferon Alpha ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between IFNα and analogues was observed.