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BlueGene Biotech Mouse Interferon Alpha ELISA kit (E03I0343)

E03I0343 Mouse Interferon Alpha ELISA kit

The Mouse Interferon Alpha ELISA kit can be used to identify samples from the mouse species. Interferon Alpha can also be called  IFNA1, IFL, LeIF D, IFN, IFN-Alpha, IFNA13, IFN-A, IFNAP22, IFN-Alpha 1b, Interferon Alpha 1b, Interferon, Leukocytic, IFN, Leukocyte, Interferon alpha-D, IFNα.

Products

Specifications of Mouse Interferon Alpha ELISA kit

Product Information

Cat. No.

E03I0343

Product Name

Mouse Interferon Alpha ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

50-1000pg/ml

Sensitivity

1.0pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/ml1 vial

STANDARD B (0.5mL)

50 pg/ml

1 vial

STANDARD C (0.5mL)

100 pg/ml

1 vial

STANDARD D (0.5mL)

250 pg/ml

1 vial

STANDARD E (0.5mL)

500 pg/ml

1 vial

STANDARD F (0.5mL)

1000 pg/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IFNα ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IFNα antibody and an IFNα-HRP conjugate. The assay sample and buffer are incubated together with IFNα-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IFNα concentration since IFNα from samples and IFNα-HRP conjugate compete for the anti-IFNα antibody binding site. Since the number of sites is limited, as more sites are occupied by IFNα from the sample, fewer sites are left to bind IFNα-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFNα concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Interferon Alpha ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IFNα and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Interferon Alpha ELISA kit

Summary of the Assay Procedure for Mouse Interferon Alpha ELISA kit

Citations of Mouse Interferon Alpha ELISA kit

E03I0343 has been referenced in the below publications:

扶正解毒化瘀方及有效部位干预RSV肺炎小鼠的作用机制研究。

Pathological and immunological characterization of bluetongue virus serotype 1 infection in type I interferons blocked immunocompetent adult mice.



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