E03I0010 Mouse Interleukin 1β ELISA kit
The Mouse Interleukin 1β ELISA kit can be used to identify samples from the mouse species. Interleukin 1β can also be called Catabolin, H1, IL-1β, Hematopoietin 1, IFN beta inducing factor, IL 1, IL 1 beta, IL 1B, IL1B, IL1F2, Interleukin 1 beta, Interleukin 1 beta precursor, LAF, OAF, Osteoclast activating factor, Preinterleukin beta, Pro interleukin 1 beta, IInterleukin-1 beta, IL 1β.
Product Information | |
Cat. No. | E03I0010 |
Product Name | Mouse Interleukin 1β ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000pg/ml |
Sensitivity | 1.0pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/ml | 1 vial |
STANDARD B (0.5mL) | 50 pg/ml | 1 vial |
STANDARD C (0.5mL) | 100 pg/ml | 1 vial |
STANDARD D (0.5mL) | 250 pg/ml | 1 vial |
STANDARD E (0.5mL) | 500 pg/ml | 1 vial |
STANDARD F (0.5mL) | 1000 pg/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IL 1β ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IL 1β antibody and an IL 1β-HRP conjugate. The assay sample and buffer are incubated together with IL 1β-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL 1β concentration since IL 1β from samples and IL 1β-HRP conjugate compete for the anti-IL 1β antibody binding site. Since the number of sites is limited, as more sites are occupied by IL 1β from the sample, fewer sites are left to bind IL 1β-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 1β concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IL 1β and analogues was observed. | |
E03I0010 has been referenced in the below publications：
TLR9在细菌DNA诱导心肌炎症反应中BNP的作用。
Role of NLRP3 inflammasome in endothelial cells inflammation induced by high uric acid.
EXPERIMENTAL STUDY OF CAFFEIC ACID COMBINED HYDROGEN-RICH NORMAL SALINE TO TREAT ACUTE RADIATION DISEASE OF BONE MARROW.
Increased inflammatory reaction in tail-suspension mice infected by E. coli in spaceglight.
Effect of medium-chain fatty acid on the expression of TLＲ4 pathway related molecules in small intestine of C57BL/6J mice with obesity.
硒化乌拉尔甘草多糖的抗炎活性研究。
Experimental study of caffeicacid combined hydrogen-rich normal saline totreat acute radiation disease of bone marrow.
Protective effect of phloretin on lupus nephritis in MRL / lpr mice via NF-κB.
Effect of caffeic acid on the expression of hematopoietic regulatory factors in lethally irradiated mice.
Atsttrin reduces lipopolysaccharide-induced neuroinflammation by inhibiting the nuclear factor kappa B signaling pathway.
Kang-Xian Pills Inhibit Inflammatory Response and Decrease Gut Permeability to Treat Carbon Tetrachloride-Induced Chronic Hepatic Injury through Modulating Gut Microbiota.
Pathological and immunological characterization of bluetongue virus serotype 1 infection in type I interferons blocked immunocompetent adult mice.
Exposure to polystyrene microplastics causes reproductive toxicity through oxidative stress and activation of the p38 MAPK signaling pathway.
Prenatal exposure to ambient fine particulate matter induces dysregulations of lipid metabolism in adipose tissue in male offspring.
Bhlhe40 deficiency attenuates LPS-induced acute lung injury through preventing macrophage pyroptosis.
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