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BlueGene Biotech Mouse Interleukin 18 ELISA kit (E03I0310)

E03I0310 Mouse Interleukin 18 ELISA kit

The Mouse Interleukin 18 ELISA kit can be used to identify samples from the mouse species. Interleukin 18 can also be called IL18, IGIF, interferon gamma-inducing factor IGIF, Interferon gamma-inducing factor, IFN-gamma-inducing factor, Interleukin-1 gamma, Iboctadekin, IFN gamma inducing factor, IL 1 gamma,  IL 1g, IL1 gamma, IL18 protein, IL1F4,  Interferon gamma inducing factor.

Products

Specifications of Mouse Interleukin 18 ELISA kit

Product Information

Cat. No.

E03I0310

Product Name

Mouse Interleukin 18 ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

50-1000pg/ml

Sensitivity

1.0pg/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IL18 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IL18. Standards or samples are then added to the microtiter plate wells and IL18 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IL18 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IL18 are added to each well to sandwichthe IL18 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL18 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL18 concentration in each sample is interpolated from this standard curve.


Quality Control on Mouse Interleukin 18 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IL18 selectin and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Interleukin 18 ELISA kit

Summary of the Assay Procedure for Mouse Interleukin 18 ELISA kit

Citations of Mouse Interleukin 18 ELISA kit

E03I0310 has been referenced in the below publications:

Therapeutic Effect and Discussion of Immunological Mechanism of Compound Lidocaine Cream on Contact Der-matitis Model of Mice.

Role of NLRP3 inflammasome in endothelial cells inflammation induced by high uric acid.

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