E03I0374 Mouse Interleukin 23 ELISA kit
The Mouse Interleukin 23 ELISA kit can be used to identify samples from the mouse species. Interleukin 23 can also be called IL 23, IL 23A, IL23P19, Interleukin 23 alpha subunit p19, JKA3 induced upon T cell activation, P19, SGRF, IL-23 subunit alpha, IL-23p19, Interleukin 23 p19 subunit.
Product Information | |
Cat. No. | E03I0374 |
Product Name | Mouse Interleukin 23 ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000pg/ml |
Sensitivity | 1.0pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/ml | 1 vial |
STANDARD B (0.5mL) | 50 pg/ml | 1 vial |
STANDARD C (0.5mL) | 100 pg/ml | 1 vial |
STANDARD D (0.5mL) | 250 pg/ml | 1 vial |
STANDARD E (0.5mL) | 500 pg/ml | 1 vial |
STANDARD F (0.5mL) | 1000 pg/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IL 23 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IL 23 antibody and an IL 23-HRP conjugate. The assay sample and buffer are incubated together with IL 23-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL 23 concentration since IL 23 from samples and IL 23-HRP conjugate compete for the anti-IL 23 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL 23 from the sample, fewer sites are left to bind IL 23-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 23 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IL 23 and analogues was observed. | |
E03I0374 has been referenced in the below publications：
The effect of Electro-Acupuncture on intestinal morphology andserum IL-23 level of Ulcerative Colitis mice model.
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