Mouse Interleukin 33 ELISA kit (E03I0204) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Mouse Interleukin 33 ELISA kit (E03I0204)

E03I0204 Mouse Interleukin 33 ELISA kit

The Mouse Interleukin 33 ELISA kit can be used to identify samples from the mouse species. Interleukin 33 can also be called DV27, C9ORF26, IL1F11, NFHEV, Interleukin-1 Family, Member 11, Nuclear factor from high endothelial venules, IL 33.

Products

Specifications of Mouse Interleukin 33 ELISA kit

Product Information
Cat. No.	E03I0204
Product Name	Mouse Interleukin 33 ELISA kit
Species	Mouse
Product Size	48 Tests / 96 Tests
Concentration	50-1000pg/ml
Sensitivity	1.0pg/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/ml	1 vial
STANDARD B (0.5mL)	50 pg/ml	1 vial
STANDARD C (0.5mL)	100 pg/ml	1 vial
STANDARD D (0.5mL)	250 pg/ml	1 vial
STANDARD E (0.5mL)	500 pg/ml	1 vial
STANDARD F (0.5mL)	1000 pg/ml	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

IL 33 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IL 33 antibody and an IL 33-HRP conjugate. The assay sample and buffer are incubated together with IL 33-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL 33 concentration since IL 33 from samples and IL 33-HRP conjugate compete for the anti-IL 33 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL 33 from the sample, fewer sites are left to bind IL 33-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 33 concentration in each sample is interpolated from this standard curve.

Quality Control on Mouse Interleukin 33 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between IL 33 and analogues was observed.