E03I0389 Mouse Interleukin 35 ELISA kit
The Mouse Interleukin 35 ELISA kit can be used to identify samples from the mouse species. Interleukin 35 can also be called IL 35.
Specifications of Mouse Interleukin 35 ELISA kit
Product Information | |
Cat. No. | E03I0389 |
Product Name | Mouse Interleukin 35 ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000pg/ml |
Sensitivity | 1.0pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/ml | 1 vial |
STANDARD B (0.5mL) | 50 pg/ml | 1 vial |
STANDARD C (0.5mL) | 100 pg/ml | 1 vial |
STANDARD D (0.5mL) | 250 pg/ml | 1 vial |
STANDARD E (0.5mL) | 500 pg/ml | 1 vial |
STANDARD F (0.5mL) | 1000 pg/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IL 35 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IL 35 antibody and an IL 35-HRP conjugate. The assay sample and buffer are incubated together with IL 35-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL 35 concentration since IL 35 from samples and IL 35-HRP conjugate compete for the anti-IL 35 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL 35 from the sample, fewer sites are left to bind IL 35-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 35 concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Interleukin 35 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IL 35 and analogues was observed. | |
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Summary of the Assay Procedure for Mouse Interleukin 35 ELISA kit
Citations of Mouse Interleukin 35 ELISA kit
E03I0389 has been referenced in the below publications：
IL-35 is elevated in clinical and experimental sepsis and mediates inflammation.
Interleukin-35 is upregulated in response to influenza virus infection and secondary bacterial pneumonia.
