E03I0007 Mouse Interleukin 4 ELISA kit
The Mouse Interleukin 4 ELISA kit can be used to identify samples from the mouse species. Interleukin 4 can also be called IL4, BSF1, BCGF1, B Cell Stimulatory Factor 1, Lymphocyte stimulatory factor 1, B Cell Growth Factor, Binetrakin, Pitrakinra.
Product Information | |
Cat. No. | E03I0007 |
Product Name | Mouse Interleukin 4 ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000pg/ml |
Sensitivity | 1.0pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/ml | 1 vial |
STANDARD B (0.5mL) | 50 pg/ml | 1 vial |
STANDARD C (0.5mL) | 100 pg/ml | 1 vial |
STANDARD D (0.5mL) | 250 pg/ml | 1 vial |
STANDARD E (0.5mL) | 500 pg/ml | 1 vial |
STANDARD F (0.5mL) | 1000 pg/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IL4 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IL4 antibody and an IL4-HRP conjugate. The assay sample and buffer are incubated together with IL4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL4 concentration since IL4 from samples and IL4-HRP conjugate compete for the anti-IL4 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL4 from the sample, fewer sites are left to bind IL4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL4 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IL4 and analogues was observed. | |
E03I0007 has been referenced in the below publications：
Therapeutic Effect and Discussion of Immunological Mechanism of Compound Lidocaine Cream on Contact Der-matitis Model of Mice.
Development of rubella virus subunit vaccine and study on the identification of the key amino acids in antibody combining sites of E1 protein.
Study on macrophage polarization and cellular immune response against fasciola gigantic and its excretory-secretory products.
Anti-asthmatic effect of Xuebijing and potential role of heme oxygenase-1 in an ovalbumin-induced murine allergic rhinitis model .
The therapeutic effects of UTI on Allergic rhinitis and the relationship with heme oxygenase-l.
乌司他丁对支气管哮喘小鼠氧化应激及血红素加氧酶1的影响。
消疹止痒喷剂对皮炎及瘙痒模型的药效学观察。
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