E03I0024 Mouse Interleukin 5 ELISA kit
The Mouse Interleukin 5 ELISA kit can be used to identify samples from the mouse species. Interleukin 5 can also be called B Cell Differentiation Factor I, IL5, IL 5, Colony stimulating factor, Colony Stimulating Factor Eosinophil, EDF, Eosinophil, Eosinophil Differentiation Factor, IL 5, Interleukin 5, B-cell differentiation factor I, IL-5, IL5_RAT, Interleukin-5, T Cell Replacing Factor, T-cell replacing factor, Interleukin5, TRF.
Product Information | |
Cat. No. | E03I0024 |
Product Name | Mouse Interleukin 5 ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000pg/ml |
Sensitivity | 1.0pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/ml | 1 vial |
STANDARD B (0.5mL) | 50 pg/ml | 1 vial |
STANDARD C (0.5mL) | 100 pg/ml | 1 vial |
STANDARD D (0.5mL) | 250 pg/ml | 1 vial |
STANDARD E (0.5mL) | 500 pg/ml | 1 vial |
STANDARD F (0.5mL) | 1000 pg/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IL 5 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IL 5 antibody and an IL 5-HRP conjugate. The assay sample and buffer are incubated together with IL 5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL 5 concentration since IL 5 from samples and IL 5-HRP conjugate compete for the anti-IL 5 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL 5 from the sample, fewer sites are left to bind IL 5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 5 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IL 5 and analogues was observed. | |
E03I0007 has been referenced in the below publications：
Development of rubella virus subunit vaccine and study on the identification of the key amino acids in antibody combining sites of E1 protein.
Anti-asthmatic effect of Xuebijing and potential role of heme oxygenase-1 in an ovalbumin-induced murine allergic rhinitis model.
Ulinastatin inhibit IL-4 mediated mast cells activation viaup-regulation of heme oxygenase-1.
乌司他丁对支气管哮喘小鼠氧化应激及血红素加氧酶1的影响。
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