E03L0230 Mouse Lactose ELISA kit
The Mouse Lactose ELISA kit can be used to identify samples from the mouse species. Lactose can also be called Anhydrous lactose, Lactosum, anhydrous, Lactosum, Lac.
Specifications of Mouse Lactose ELISA kit
Product Information | |
Cat. No. | E03L0230 |
Product Name | Mouse Lactose ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000pg/ml |
Sensitivity | 1.0pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/ml | 1 vial |
STANDARD B (0.5mL) | 50 pg/ml | 1 vial |
STANDARD C (0.5mL) | 100 pg/ml | 1 vial |
STANDARD D (0.5mL) | 250 pg/ml | 1 vial |
STANDARD E (0.5mL) | 500 pg/ml | 1 vial |
STANDARD F (0.5mL) | 1000 pg/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Lac ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-Lac antibody and an Lac-HRP conjugate. The assay sample and buffer are incubated together with Lac-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Lac concentration since Lac from samples and Lac-HRP conjugate compete for the anti-Lac antibody binding site. Since the number of sites is limited, as more sites are occupied by Lac from the sample, fewer sites are left to bind Lac-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Lac concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Lactose ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Lac and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Lactose ELISA kit
Citations of Mouse Lactose ELISA kit
E03L0230 has been referenced in the below publications：
Environmental noise stress disturbs commensal microbiota homeostasis and induces oxi-inflammmation and AD-like neuropathology through epithelial barrier disruption in the EOAD mouse model.
