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  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Leukotriene C4 ELISA kit (E03L0036)

E03L0036 Mouse Leukotriene C4 ELISA kit

The Mouse Leukotriene C4 ELISA kit can be used to identify samples from the mouse species. Leukotriene C4 can also be called LT-C4, LTC4.

Products

Specifications of Mouse Leukotriene C4 ELISA kit

Product Information

Cat. No.

E03L0036

Product Name

Mouse Leukotriene C4 ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

50-1000pg/ml

Sensitivity

1.0pg/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

LTC4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for LTC4. Standards or samples are then added to the microtiter plate wells and LTC4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of LTC4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for LTC4 are added to each well to sandwichthe LTC4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain LTC4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LTC4 concentration in each sample is interpolated from this standard curve.


Quality Control on Mouse Leukotriene C4 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between LTC4 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Leukotriene C4 ELISA kit

Summary of the Assay Procedure for Mouse Leukotriene C4 ELISA kit

Citations of Mouse Leukotriene C4 ELISA kit

E03L0036 has been referenced in the below publications:

Ameliorative effect of selective NLRP3 inflammasome inhibitor MCC950 in an ovalbumin-induced allergic rhinitis murine model.

Therapeutic effects of SKF-96365 on murine allergic rhinitis induced by OVA.

Downregulation of Orai1 expression in the airway alleviates murine allergic rhinitis.

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