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  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Liver Kidney Microsome Autoantibody ELISA kit (E03L0238)

E03L0238 Mouse Liver Kidney Microsome Autoantibody ELISA kit

The Mouse Liver Kidney Microsome Autoantibody ELISA kit can be used to identify samples from the mouse species. Liver Kidney Microsome Autoantibody can also be called PLA2G7, PAF-AH, PAFAH, Lp-PLA2, LDL-PLA2, Platelet Activating Factor Acetylhydrolase, Plasma, Phospholipase A2, Group VII, LDL-associated phospholipase A2, Phospholipase A2, Lipoprotein Associated, LKM.

Products

Specifications of Mouse Liver Kidney Microsome Autoantibody ELISA kit

Product Information

Cat. No.

E03L0238

Product Name

Mouse Liver Kidney Microsome Autoantibody ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

5.0-100ng/ml

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

LKM ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-LKM antibody and an LKM-HRP conjugate. The assay sample and buffer are incubated together with LKM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LKM concentration since LKM from samples and LKM-HRP conjugate compete for the anti-LKM antibody binding site. Since the number of sites is limited, as more sites are occupied by LKM from the sample, fewer sites are left to bind LKM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LKM concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Liver Kidney Microsome Autoantibody ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between LKM and analogues was observed.


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Summary of the Assay Procedure for Mouse Liver Kidney Microsome Autoantibody ELISA kit

Summary of the Assay Procedure for Mouse Liver Kidney Microsome Autoantibody ELISA kit

Citations of Mouse Liver Kidney Microsome Autoantibody ELISA kit

E03L0238 has been referenced in the below publications:

Medullary thymic epithelial cell depletion leads to autoimmune hepatitis.



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