E03L0238 Mouse Liver Kidney Microsome Autoantibody ELISA kit
The Mouse Liver Kidney Microsome Autoantibody ELISA kit can be used to identify samples from the mouse species. Liver Kidney Microsome Autoantibody can also be called PLA2G7, PAF-AH, PAFAH, Lp-PLA2, LDL-PLA2, Platelet Activating Factor Acetylhydrolase, Plasma, Phospholipase A2, Group VII, LDL-associated phospholipase A2, Phospholipase A2, Lipoprotein Associated, LKM.
Product Information | |
Cat. No. | E03L0238 |
Product Name | Mouse Liver Kidney Microsome Autoantibody ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100ng/ml |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
LKM ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-LKM antibody and an LKM-HRP conjugate. The assay sample and buffer are incubated together with LKM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LKM concentration since LKM from samples and LKM-HRP conjugate compete for the anti-LKM antibody binding site. Since the number of sites is limited, as more sites are occupied by LKM from the sample, fewer sites are left to bind LKM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LKM concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between LKM and analogues was observed. | |
E03L0238 has been referenced in the below publications：
Medullary thymic epithelial cell depletion leads to autoimmune hepatitis.
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