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BlueGene Biotech Mouse Myeloperoxidase ELISA kit (E03M0032)

E03M0032 Mouse Myeloperoxidase ELISA kit

The Mouse Myeloperoxidase ELISA kit can be used to identify samples from the mouse species. Myeloperoxidase can also be called MPO, 89 kDa myeloperoxidase, 84 kDa myeloperoxidase, Myeloperoxidase heavy chain, PERM.

Products

Specifications of Mouse Myeloperoxidase ELISA kit

Product Information

Cat. No.

E03M0032

Product Name

Mouse Myeloperoxidase ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

5.0-100ng/ml

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

MPO ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MPO antibody and an MPO-HRP conjugate. The assay sample and buffer are incubated together with MPO-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MPO concentration since MPO from samples and MPO-HRP conjugate compete for the anti-MPO antibody binding site. Since the number of sites is limited, as more sites are occupied by MPO from the sample, fewer sites are left to bind MPO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MPO concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Myeloperoxidase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between MPO and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Myeloperoxidase ELISA kit

Summary of the Assay Procedure for Mouse Myeloperoxidase ELISA kit

Citations of Mouse Myeloperoxidase ELISA kit

E03M0032 has been referenced in the below publications:

Pycnogenol, a compound isolated from the bark of pinus maritime mill, attenuates ventilator-induced lung injury through inhibiting NF-κB-mediated inflammatory response.

The Role of IL-33 on LPS-Induced Acute Lung Injury in Mice.

Nanocurcumin ameliorates Staphylococcus aureus-induced mastitis in mouse by suppressing NF‑κB signaling and inflammation.


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