Mouse Platelet Activating Factor Acetylhydrolase ELISA kit (E03P0573)

BlueGene Biotech Mouse Platelet Activating Factor Acetylhydrolase ELISA kit (E03P0573)

E03P0573 Mouse Platelet Activating Factor Acetylhydrolase ELISA kit

The Mouse Platelet Activating Factor Acetylhydrolase ELISA kit can be used to identify samples from the mouse species. Platelet Activating Factor Acetylhydrolase can also be called PAF AH, SD-PLA, Serine-dependent phospholipase A, Platelet-activating factor acetylhydrolase, cytoplasmic.

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Specifications of Mouse Platelet Activating Factor Acetylhydrolase ELISA kit

Product Information
Cat. No.	E03P0573
Product Name	Mouse Platelet Activating Factor Acetylhydrolase ELISA kit
Species	Mouse
Product Size	48 Tests / 96 Tests
Concentration	2.5-50 ng/mL
Sensitivity	0.1 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	2.5 ng/mL	1 vial
STANDARD C (0.5mL)	5 ng/mL	1 vial
STANDARD D (0.5mL)	10 ng/mL	1 vial
STANDARD E (0.5mL)	25 ng/mL	1 vial
STANDARD F (0.5mL)	50 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

PAF AH ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PAF AH antibody and an PAF AH-HRP conjugate. The assay sample and buffer are incubated together with PAF AH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PAF AH concentration since PAF AH from samples and PAF AH-HRP conjugate compete for the anti-PAF AH antibody binding site. Since the number of sites is limited, as more sites are occupied by PAF AH from the sample, fewer sites are left to bind PAF AH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAF AH concentration in each sample is interpolated from this standard curve.

Quality Control on Mouse Platelet Activating Factor Acetylhydrolase ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between PAF AH and analogues was observed.

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Summary of the Assay Procedure for Mouse Platelet Activating Factor Acetylhydrolase ELISA kit

Citations of Mouse Platelet Activating Factor Acetylhydrolase ELISA kit

E03P0573 has been referenced in the below publications：

Triglyceride with medium-chain fatty acids increases the activity and expression of hormone-sensitive lipase in white adipose tissue of C57BL/6J mice.