Mouse Receptor Activator of Nuclear Factor Kb Ligand ELISA kit (E03R0005)

BlueGene Biotech Mouse Receptor Activator of Nuclear Factor Kb Ligand ELISA kit (E03R0005)

E03R0005 Mouse Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

The Mouse Receptor Activator of Nuclear Factor Kb Ligand ELISA kit can be used to identify samples from the mouse species. Receptor Activator of Nuclear Factor Kb Ligand can also be called RANKL, CD254, TNFSF11, ODF, OPGL, TRANCE, HRANKL2, SOdf, Tumor Necrosis Factor(ligand)superfamily Member 11, TNF-related activation-induced cytokine.

Products

Specifications of Mouse Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

Product Information
Cat. No.	E03R0005
Product Name	Mouse Receptor Activator of Nuclear Factor Kb Ligand ELISA kit
Species	Mouse
Product Size	48 Tests / 96 Tests
Concentration	50-1000pg/ml
Sensitivity	1.0pg/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/ml	1 vial
STANDARD B (0.5mL)	50 pg/ml	1 vial
STANDARD C (0.5mL)	100 pg/ml	1 vial
STANDARD D (0.5mL)	250 pg/ml	1 vial
STANDARD E (0.5mL)	500 pg/ml	1 vial
STANDARD F (0.5mL)	1000 pg/ml	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

RANKL ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-RANKL antibody and an RANKL-HRP conjugate. The assay sample and buffer are incubated together with RANKL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RANKL concentration since RANKL from samples and RANKL-HRP conjugate compete for the anti-RANKL antibody binding site. Since the number of sites is limited, as more sites are occupied by RANKL from the sample, fewer sites are left to bind RANKL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RANKL concentration in each sample is interpolated from this standard curve.

Quality Control on Mouse Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between RANKL and analogues was observed.

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Summary of the Assay Procedure for Mouse Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

Citations of Mouse Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

E03R0005 has been referenced in the below publications：

Studies on the anti-osteoporosis effective substances and quality control of Eucommia ulmoides based on MC3T3-E1 cell.

Low-Dose Staphylococcal Enterotoxin C2 Mutant Maintains Bone Homeostasis via Regulating Crosstalk between Bone Formation and Host T-Cell Eﬀector Immunity.