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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Secretory Immunoglobulin A ELISA kit (E03S0003)

E03S0003 Mouse Secretory Immunoglobulin A ELISA kit

The Mouse Secretory Immunoglobulin A ELISA kit can be used to identify samples from the mouse species. Secretory Immunoglobulin A can also be called SIgA, secretory IgA.

Products

Specifications of Mouse Secretory Immunoglobulin A ELISA kit

Product Information

Cat. No.

E03S0003

Product Name

Mouse Secretory Immunoglobulin A ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

100-2500mg/L

Sensitivity

1.0mg/L

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 mg/L

1 vial

STANDARD B (0.5mL)

100 mg/L

1 vial

STANDARD C (0.5mL)

250 mg/L

1 vial

STANDARD D (0.5mL)

500 mg/L

1 vial

STANDARD E (0.5mL)

1000 mg/L

1 vial

STANDARD F (0.5mL)

2500 mg/L

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SIgA ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-SIgA antibody and an SIgA-HRP conjugate. The assay sample and buffer are incubated together with SIgA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SIgA concentration since SIgA from samples and SIgA-HRP conjugate compete for the anti-SIgA antibody binding site. Since the number of sites is limited, as more sites are occupied by SIgA from the sample, fewer sites are left to bind SIgA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SIgA concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Secretory Immunoglobulin A ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SIgA and analogues was observed.


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Summary of the Assay Procedure for Mouse Secretory Immunoglobulin A ELISA kit

Summary of the Assay Procedure for Mouse Secretory Immunoglobulin A ELISA kit

Citations of Mouse Secretory Immunoglobulin A ELISA kit

E03S0003 has been referenced in the below publications:

Cryptosporidium parvum. Protective Immunity against Cryptosporidiosis with Recombinant Lactobacillus Live Vaccines Expressing cp966 after Oral Immunization.

Influence on gastric mucosa specific antibody in newborn mice after oral immunization with inactived H. pylori.

Study on the pathogenicity of UU1, UU3 and UU4 in the lower genital tract of female KM mice.


 


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