E03S0291 Mouse Soluble Transferrin Receptor ELISA kit
The Mouse Soluble Transferrin Receptor ELISA kit can be used to identify samples from the mouse species. Soluble Transferrin Receptor can also be called sCD71, sTFR1, sP90, sTFRC, sT9, sTrfr, sTfR, sTransferrin receptor protein 1, serum form.
Product Information | |
Cat. No. | E03S0291 |
Product Name | Mouse Soluble Transferrin Receptor ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100ng/ml |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
sTfR ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-sTfR antibody and an sTfR-HRP conjugate. The assay sample and buffer are incubated together with sTfR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sTfR concentration since sTfR from samples and sTfR-HRP conjugate compete for the anti-sTfR antibody binding site. Since the number of sites is limited, as more sites are occupied by sTfR from the sample, fewer sites are left to bind sTfR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sTfR concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between sTfR and analogues was observed. | |
E03S0291 has been referenced in the below publications：
n-Butanol soluble fraction of the water extract of Chinese toon fruit ameliorated focal brain ischemic insult in rats via inhibition of oxidative stress and inflammation.
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