E03S0006 Mouse Stromal Cell Derived Fctor 1Alpha ELISA kit
The Mouse Stromal Cell Derived Fctor 1Alpha ELISA kit can be used to identify samples from the mouse species. Stromal Cell Derived Fctor 1Alpha can also be called CXCL12, SDF-1, and SDF1Alpha．
Product Information | |
Cat. No. | E03S0006 |
Product Name | Mouse Stromal Cell Derived Fctor 1Alpha ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10ng/ml |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/ml | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/ml | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/ml | 1 vial |
STANDARD E (0.5mL) | 5.0ng/ml | 1 vial |
STANDARD F (0.5mL) | 10 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
SDF1Alpha ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-SDF1Alpha antibody and a SDF1Alpha -HRP conjugate. The assay sample and buffer are incubated together with SDF1Alpha -HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SDF1Alpha concentration since SDF1Alpha from samples and SDF1Alpha -HRP conjugate compete for the anti-SDF1Alpha antibody binding site. Since the number of sites is limited, as more sites are occupied by SDF1Alpha from the sample, fewer sites are left to bind SDF1Alpha -HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SDF1Alpha concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between SDF1Alpha and analogues was observed. | |
E03S0006 has been referenced in the below publications：
Reverse-D-4F Increases the Number of Endothelial Progenitor Cells and Improves Endothelial Progenitor Cell Dysfunctions in High Fat Diet Mice.
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