E03T0069 Mouse Toll Like Receptor4 ELISA kit
The Mouse Toll Like Receptor4 ELISA kit can be used to identify samples from the mouse species. Toll Like Receptor4 can also be called TLR4, ARMD10, CD284, TLR-4, TOLL, Toll Like Receptor 4.
Specifications of Mouse Toll Like Receptor4 ELISA kit
Product Information | |
Cat. No. | E03T0069 |
Product Name | Mouse Toll Like Receptor4 ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10ng/ml |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/ml | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/ml | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/ml | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/ml | 1 vial |
STANDARD F (0.5mL) | 10 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TLR4 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TLR4 antibody and a TLR4-HRP conjugate. The assay sample and buffer are incubated together with TLR4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TLR4 concentration since TLR4 from samples and TLR4-HRP conjugate compete for the anti-TLR4 antibody binding site. Since the number of sites is limited, as more sites are occupied by TLR4 from the sample, fewer sites are left to bind TLR4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TLR4 concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Toll Like Receptor4 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TLR4 and analogues was observed. | |
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Summary of the Assay Procedure for Mouse Toll Like Receptor4 ELISA kit
Citations of Mouse Toll Like Receptor4 ELISA kit
E03T0069 has been referenced in the below publications：
Sitagliptin ameliorates thioacetamide-induced acute liver injury via modulating TLR4/NF-KB signaling pathway in mice.
