E03T0034 Mouse Total Tumor Necrosis Factor ELISA kit
The Mouse Total Tumor Necrosis Factor ELISA kit can be used to identify samples from the mouse species. Total Tumor Necrosis Factor can also be called TNF, DIF, TNF-alpha, TNFA, TNFSF2, Tumour necrosis factor, TNF-伪, tumor necrosis factor, TNLG1F, Tumor necrosis factor alpha．
Product Information | |
Cat. No. | E03T0034 |
Product Name | Mouse Total Tumor Necrosis Factor ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000pg/ml |
Sensitivity | 1.0pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/ml | 1 vial |
STANDARD B (0.5mL) | 50 pg/ml | 1 vial |
STANDARD C (0.5mL) | 100 pg/ml | 1 vial |
STANDARD D (0.5mL) | 250 pg/ml | 1 vial |
STANDARD E (0.5mL) | 500 pg/ml | 1 vial |
STANDARD F (0.5mL) | 1000 pg/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TNF ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TNF antibody and an TNF-HRP conjugate. The assay sample and buffer are incubated together with TNF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TNF concentration since TNF from samples and TNF-HRP conjugate compete for the anti-TNF antibody binding site. Since the number of sites is limited, as more sites are occupied by TNF from the sample, fewer sites are left to bind TNF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TNF concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TNF and analogues was observed. | |
E03T0034 has been referenced in the below publications：
The study of biocompatibility of pristine and functionalized multi-walled carbon nanotubes.
Environmental noise stress disturbs commensal microbiota homeostasis and induces oxi-inflammmation and AD-like neuropathology through epithelial barrier disruption in the EOAD mouse model.
Short-and long-term toxicities of multi-walled carbon nanotubes in vivo and in vitro.
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