E03T0008 Mouse Tumor Necrosis Factor Alpha ELISA kit
The Mouse Tumor Necrosis Factor Alpha ELISA kit can be used to identify samples from the mouse species. Tumor Necrosis Factor Alpha can also be called TNF-α, DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2．
Product Information | |
Cat. No. | E03T0008 |
Product Name | Mouse Tumor Necrosis Factor Alpha ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000pg/ml |
Sensitivity | 1.0pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/ml | 1 vial |
STANDARD B (0.5mL) | 50 pg/ml | 1 vial |
STANDARD C (0.5mL) | 100 pg/ml | 1 vial |
STANDARD D (0.5mL) | 250 pg/ml | 1 vial |
STANDARD E (0.5mL) | 500 pg/ml | 1 vial |
STANDARD F (0.5mL) | 1000 pg/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TNF-α ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TNF-α antibody and an TNF-α-HRP conjugate. The assay sample and buffer are incubated together with TNF-α-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TNF-α concentration since TNF-α from samples and TNF-α-HRP conjugate compete for the anti-TNF-α antibody binding site. Since the number of sites is limited, as more sites are occupied by TNF-α from the sample, fewer sites are left to bind TNF-α-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TNF-α concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TNF-α and analogues was observed. | |
E03T0008 has been referenced in the below publications：
Triacetyl-3-hydroxyphenyladenosine, a derivative of cordycepin,attenuates atherosclerosis in apolipoprotein E-knockout mice.
Effect of lipid-bound apolipoprotein A-I cysteinemutant on ATF3 in RAW264.7 cells.
TLR9在细菌DNA诱导心肌炎症反应中BNP的作用。
EXPERIMENTAL STUDY OF CAFFEIC ACID COMBINED HYDROGEN-RICH NORMAL SALINE TO TREAT ACUTE RADIATION DISEASE OF BONE MARROW.
Correlation between myocardial injury and expression of TNF-a on myocardium tissue of mice induced by agkistrodon halys pallas venom.
The study of Sagittaria sagittifolia polysaccharide about its regulating immune function on mice and its possible mechanism on mTOR signaling pathway .
THE PRELIMINARILYSTUDYOFANTI-TUMOR EFFECT AND ITS MOLECULAR MECHANISMS OF CALCULUS BOVIS CULTIVATED BY GLUCURONIDASE.
Effects of Plantain Seed Polysaccharide on anti-inflammatory and anti-tumor.
Effect of common edible oils on lipid metabolism and its mechanism in obese mice induced by a high fat diet.
Increased inflammatory reaction in tail-suspension mice infected by E. coli in spaceglight.
Effects of Calculus Bovis Cultivated by Glucurornidase on Functionality of HighDensity Lipoprotein in Mice Fed with High Cholesterol Diet.
Ulinastatin inhibit IL-4 mediated mast cells activation viaup-regulation of heme oxygenase-1.
Experimental study of Anti-psoriasis decoction in treating psoriasis animal models.
THE PRELIMINARILY STUDY OF ANTI-TUMOR EFFECT AND ITS MOLECULAR MECHANISMS OF CALCULUS BOVIS CULTIVATED BY GLUCURONIDASE.
The effect of CORM-2 on pulmonary fibrosis of aged mice induced by paraquat and its mechanism MENG.
Enhancement effect of Sagittaria sagittifolia polysaccharide on immune function of macrophages through mTOＲ signal pathway.
Effect of medium-chain fatty acid on the expression of TLＲ4 pathway related molecules in small intestine of C57BL/6J mice with obesity.
Effects of Ethanol Componentons from Aconitum vilmorinianum on Inflammatory Cytokines in LPS-stimulated RAW 264.7 Cell.
Protective effect of pioglitazone on lung injury induced by irradiation.
The Effects of Endurance Exercise and Adiponectin Receptor Agonist AdipoRon on Adipose Tissue Inflammation in High Fat Diet Mice.
Regulating effect of berberine on macrophage phenotype transformation in hepatic tissue of mice with methionine-choline deficiency diet induced non-alcoholic steatohepatitis.
硒化乌拉尔甘草多糖的抗炎活性研究。
The relationship between the subtypes of functional dyspepsia and TCM differentiation and the level of GLP-1.
Experimental study of caffeicacid combined hydrogen-rich normal saline totreat acute radiation disease of bone marrow.
Protective effect of phloretin on lupus nephritis in MRL / lpr mice via NF-κB.
Study on the pathogenicity of UU1, UU3 and UU4 in the lower genital tract of female KM mice.
Effect of caffeic acid on the expression of hematopoietic regulatory factors in lethally irradiated mice.
Atsttrin reduces lipopolysaccharide-induced neuroinflammation by inhibiting the nuclear factor kappa B signaling pathway.
Kang-Xian Pills Inhibit Inflammatory Response and Decrease Gut Permeability to Treat Carbon Tetrachloride-Induced Chronic Hepatic Injury through Modulating Gut Microbiota.
Pathological and immunological characterization of bluetongue virus serotype 1 infection in type I interferons blocked immunocompetent adult mice.
Follistatin-like protein 1 suppressed pro-inflammatory cytokines expression during neuroinflammation induced by lipopolysaccharide.
Quercetin and lithium chloride potentiate the protective effects of carvedilol against renal ischemia-reperfusion injury in high-fructose, high-fat diet-fed Swiss albino mice independent of renal lipid signaling.
Prenatal exposure to ambient fine particulate matter induces dysregulations of lipid metabolism in adipose tissue in male offspring.
Reverse-D-4F Increases the Number of Endothelial Progenitor Cells and Improves Endothelial Progenitor Cell Dysfunctions in High Fat Diet Mice.
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