NE03I0431 Mouse Immunoglobulin G ELISA kit
Mouse Immunoglobulin G ELISA kit is suitable for the detection of samples from mouse species. Immunoglobulin G can also be called as IgG.
Specifications of Mouse Immunoglobulin G ELISA kit
Product Information | |
Cat. NO. | NE03I0431 |
Product Name | Mouse Immunoglobulin G ELISA kit |
Species | Mouse |
Product Size | 96 Tests |
Concentration | 78.125-5000 pg/ml |
Sensitivity | 29.3 pg/ml |
Principal | Sandwich ELISA |
Sample Volume | 100 ul |
Sample Type | ELISA Kit for the quantitative Measurement of mouse IgG Residues in Protein Purification Process, and End-Product (purified fermentation broth, cell culture supernatant, etc.) |
Assay Time | 3 hours and 15 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
Reagents | Specification | Quantity |
Pre-Coated Microplate (Detachable) | 96 wells | 1 plate (Keep Sealed) |
| Standard (Stock Solution) -5ug/ml | 20ul | 1 vial |
Standard S1 | 5000pg/ml | 1 vial |
Standard S2 | 2500pg/ml | 1 vial |
Standard S3 | 1250pg/ml | 1 vial |
Standard S4 | 625pg/ml | 1 vial |
Standard S5 | 312.5pg/ml | 1 vial |
Standard S6 | 156.25pg/ml | 1 vial |
Standard S7 | 78.125pg/ml | 1 vial |
Detection antibody (100×) | 150ul | 1 vial |
HRP-conjugated antibody(200×) | 100ul | 1 vial |
Diluent Buffer (10×) | 10ml | 1 vial |
TMB Substrates | 10ml | 1 vial (Avoid Light) |
Stop Solution | 10ml | 1 vial |
Wash Solution (100×) | 10ml | 1 vial |
Plate Sealer | 4 pieces | |
Instruction Manual | 1 | |
Principle of the Assay |
Mouse immunoglobulin G (IgG) is the main component of immunoglobulin in serum, it is mainly created and secreted by plasma cells in spleen and lymph nodes and exists in the form of monomer. IgG comprises approximately 75% of serum immunoglobulin. Its normal value is in mg/ml in body fluids with 40%-50% distributed in serum and the rest distributed in tissues. Immunoglobulin in the immune serum of human and animals has very dissimilar concentration and structure, there is considerable discrepancy with their composition, structure, size, electric charge, and biological activity, etc. The immunoglobulin comprises about 20-25% of whole serum albumin. To date, 5 types of immunoglobulin have been isolated and purified in human, mouse and other animals' serum-IgG, IgM, IgA, IgD and IgE. According to the antigenic specificity of γ chain in IgG molecule, mouse IgG is divided into 4 subtypes: IgG1, IgG2a, IgG2b and IgG3. Mouse IgG can activate complement through classical pathway, its ability to bind complement is IgG2b＞IgG2a＞IgG3, while IgG1 has no ability to bind complement. |
Quality Control on Mouse Immunoglobulin G ELISA kit
Coefficient of Variance | Intra Variation: 0.28-2.39% | ||
Inter Variation: 0.27-2.92% | |||
Recovery | 91.9-112% (10% FBS) | ||
Linearity | Diluent Ratio | Range % | Average Linearity % |
1:5×105 | 98.95 | 99.98 | |
1:10×105 | 99.7 | 100.1 | |
1:20×105 | 101 | 100.4 | |
Specificity/ Cross-reactivity | Sample | Cross-Reactivity（%） | |
Bovine IgG | 0.001 | ||
Goat IgG | --- | ||
Human IgG | --- | ||
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Summary of the Assay Procedure for Mouse Immunoglobulin G ELISA kit
Citations of Mouse Immunoglobulin G ELISA kit
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