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BlueGene Biotech Protein A ELISA kit (NEGES0890)

NEGES0890 Protein A ELISA kit

Protein A ELISA kit is suitable for the detection of samples from general species.

Products

Specifications of Protein A ELISA kit

Product Information

Cat. NO.

NEGES0890

Product Name

Protein A ELISA kit

Species

General

Product Size

96 Tests

Concentration

3.125-200 pg/ml

Sensitivity

1.4pg/ml

Principal

Sandwich ELISA

Sample Volume

100 ul

Sample Type

ELISA Kit for the quantitative Measurement of Protein A Residues in Protein

Purification Process, and End-Product (purified fermentation broth, cell culture

supernatant, etc.)

Assay Time

3 hours

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

Reagents

Specification

Quantity

Pre-Coated Microplate (Detachable)

96 wells

1 plate (Keep Sealed)

Standard (Stock Solution) -2ug/ml10ul
1 vial

Standard S1

200pg/ml

1 vial

Standard S2

100pg/ml

1 vial

Standard S3

50pg/ml

1 vial

Standard S4

25pg/ml

1 vial

Standard S5

12.5pg/ml

1 vial

Standard S6

6.25pg/ml

1 vial

Standard S7

3.125pg/ml

1 vial

HRP-conjugated antibody(200×)

100ul

1 vial

TMB Substrates

10ml

1 vial (Avoid Light)

Stop Solution

10ml

1 vial

Wash Solution (100×)

10ml

1 vial

Diluent Buffer(10x)10ml1 vial
Diluent Ingredient0.5g1 tube

Plate Sealer


4 pieces

Instruction Manual


1


Principle of the Assay

This ELISA kit is applied to the double antibody sandwich Enzyme Linked Immunosorbent Assay to detect the concentration of protein A in samples. The microtiter plate has been pre-coated with chicken anti-protein A antibody , standards or samples are then added to the microtiter plate wells and protein A if present, will bind to the antibody pre-coated wells under specific conditions. After washing, then a Horseradish Peroxidase(HRP)-conjugated detection rabbit anti-protein A antibody is added to the wells, under 37℃ incubation, to form a precoated antibody-protein A-detection antibody sandwich complex. Then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP, and finally turns to yellow immediately after adding the stop solution. Color of TMB substrate positively correlated with total protein A bound in the initial steps. The color is measured by spectrophotometrically with wavelength of 450nm. The concentration of protein A in samples is then determined by comparing the O.D. of the samples to the standard curve.


Quality Control on Protein A ELISA kit

Coefficient of Variance

Intra Variation: 4-8%

Inter Variation: 8-10%

Recovery

67.36-115.92%

Linearity

Diluent Ratio

Range %

Average Linearity %

4

98.25-105.77

102.01

40

100.68-109.74

105.21

Specificity/

Cross-reactivity

Sample

Cross-Reactivity(%)

Bovine IgG

---

Goat IgG

---

Human IgG

---


BlueGene Biotech Product Show

Summary of the Assay Procedure for Protein A ELISA kit

Summary of the Assay Procedure for Protein A ELISA kit

Citations of Protein A ELISA Kit

Not available

Product: Not available

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