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  • tmao elisa kit
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BlueGene Biotech PGEU0001 SUMO protease (Ulp1) protein, Recombinant

SUMO protease can also be called as NIB1, Ulp1.

Products

Specifications Of PGEU0001 Ulp1 (SUMO protease 1), Recombinant

Product Information

Catalog Number

PGEU0001

Package Size

10ug/50ug/500ug/1mg

Other Names

NIB1; Ulp1

Protein & NCBI Number

Q02724, NM_001183834.1

Host

E.coli

Express Region

Lys401-Lys621

Protein Sequence

KKLVPELNEKDDDQVQKALASRENTQLMNRDNIEITVRDFKTLAPRRWLNDTIIEFFMKYIE
KSTPNTVAFNSFFYTNLSERGYQGVRRWMKRKKTQIDKLDKIFTPINLNQSHWALGIIDLKK
KTIGYVDSLSNGPNAMSFAILTDLQKYVMEESKHTIGEDFDLIHLDCPQQPNGYDCGIYVCM
NTLYGSADAPLDFDYKDAIRMRRFIAHLILTDALK

Molecular Weight

The protein consists of 230 amino acids (including the fusion tag), with a
predicted molecular weight of 26.9kDa, which matches the actual molecular weight.

Fusion Tag

6×His(C-terminus)

Purity

≥85% SDS-PAGE

Physical Property

Liquid

Components0.01M PBS+20% glycerol, sterile solution

Storage & Stability

After aliquoting, the stability of the samples can be maintained for up to 6
months at -20°C to -80°C, avoiding repeated freeze-thaw cycles.

Applications

Antibody preparation, immunoassay (ELISA, WB), Cleaves the SUMO tag at the
N terminus of the fusion protein, etc.

Lead Time

5 to 10 business days;

2 to 3 days for stock products


Background

ULP1 also known as Ubiquitin-like-specific protease 1,Smt3-protein conjugate proteinase,Ulp1 endopeptidase, it is mainly sourced Saccharomyces cerevisiae (strain ATCC 204508/S288c), it includes three domains: ULP1, Peptidase-C48 and PLN03189.

Catalytic Activity: Hydrolysis of the alpha-linked peptide bond in the sequence Gly-Gly-|-Ala-Thr-Tyr at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptide, Smt3, leading to the mature form of the protein. A second reaction involves the cleavage of an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein.


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