Porcine Major Histocompatibility Complex class II Antigen ELISA kit (E07M0277)

BlueGene Biotech Porcine Major Histocompatibility Complex class II Antigen ELISA kit (E07M0277)

E07M0277 Porcine Major Histocompatibility Complex class II Antigen ELISA kit

The Porcine Major Histocompatibility Complex class II Antigen ELISA kit can be used to identify samples from the porcine species. Major Histocompatibility Complex class II Antigen can also be called MHC2, HLA-DMB, HLA class II histocompatibility antigen, DM beta chain, HLA-DMβ, DMA, DMB, DP beta 1, DPB 1, DPB1, DRB, HLA class II histocompatibility antigen DM beta chain, HLA DMB, HLA DPB1, HLADM, HLADP1B.

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Specifications of Porcine Major Histocompatibility Complex class II Antigen ELISA kit

Product Information
Cat. No.	E07M0277
Product Name	Porcine Major Histocompatibility Complex class II Antigen ELISA kit
Species	Porcine
Product Size	48 Tests / 96 Tests
Concentration	5-100 ng/mL
Sensitivity	1.0 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	5 ng/mL	1 vial
STANDARD C (0.5mL)	10 ng/mL	1 vial
STANDARD D (0.5mL)	25 ng/mL	1 vial
STANDARD E (0.5mL)	50 ng/mL	1 vial
STANDARD F (0.5mL)	100 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

MHC2 ELISA kit uses an anti-MHC2 antibody and an MHC2-HRP conjugate in a competitive enzyme immunoassay method. MHC2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MHC2 antibody binding site between MHC2 from samples and MHC2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MHC2. Since the number of sites is limited, as more sites are occupied by MHC2 from the sample, fewer sites are left to bind MHC2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MHC2 concentration in each sample is interpolated from this standard curve.

Quality Control on Porcine Major Histocompatibility Complex class II Antigen ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between MHC2 and analogues was observed.

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Summary of the Assay Procedure for Porcine Major Histocompatibility Complex class II Antigen ELISA kit

Citations of Porcine Major Histocompatibility Complex class II Antigen ELISA kit

E07M0277 has been referenced in the below publications：

Dietary fibre affects intestinal mucosal barrier function and regulates intestinal bacteria in weaning piglets.