E07U0022 Porcine UL16 Binding protein 1 ELISA kit
The Porcine UL16 Binding protein 1 ELISA kit can be used to identify samples from the porcine species. UL16 Binding protein 1 can also be called ALCAN-beta, N2DL-1, N2DL1, NKG2D ligand 1 precursor, NKG2DL1, RAET1I, Retinoic acid early transcript 1I, UL16, UL16 binding protein 1, UL16-binding protein 1.
Product Information | |
Cat. No. | E07U0022 |
Product Name | Porcine UL16 Binding protein 1 ELISA kit |
Species | Porcine |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 250 pg/mL | 1 vial |
STANDARD C (0.5mL) | 500 pg/mL | 1 vial |
STANDARD D (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD E (0.5mL) | 2500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 5000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ULBP1 ELISA kit uses an anti-ULBP1 antibody and an ULBP1-HRP conjugate in a competitive enzyme immunoassay method. ULBP1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ULBP1 antibody binding site between ULBP1 from samples and ULBP1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ULBP1. Since the number of sites is limited, as more sites are occupied by ULBP1 from the sample, fewer sites are left to bind ULBP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ULBP1 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ULBP1 and analogues was observed. | |
E07U0022 has been referenced in the below publications：
The production of UL16‑binding protein 1 targeted pigs using CRISPR technology.
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