Name: Rabbit Cluster of differentiation 63 ELISA kit (E04C1492)
Brand: Shanghai BlueGene Biotech CO., LTD.
Price: 100 USD

BlueGene Biotech Rabbit Cluster of differentiation 63 ELISA kit (E04C1492)

E04C1492 Rabbit Cluster of differentiation 63 ELISA kit

Rabbit Cluster of differentiation 63 ELISA kit is suitable for detecting samples from rabbit species. Cluster of differentiation 63 can also be called TSPAN30, LAMP-3, ME491, MLA1, OMA81H, Melanoma 1 antigen, Granulophysin, Lysosomal-associated membrane protein 3, Ocular melanoma-associated antigen, CD63 antigen, CD63 antigen (melanoma 1 antigen), granulophysin, lysosome-associated membrane glycoprotein 3, melanoma 1 antigen, melanoma-associated antigen ME491, CD63, CD 63, CD-63.

Products

Rabbit Cluster of differentiation 63 ELISA kit

Product Information
Cat. No.	E04C1492
Product Name	Rabbit Cluster of differentiation 63 ELISA kit
Species	Rabbit
Product Size	48 Tests / 96 Tests
Concentration	0.5-10ng/mL
Sensitivity	0.1 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	0.5 ng/mL	1 vial
STANDARD C (0.5mL)	1.0 ng/mL	1 vial
STANDARD D (0.5mL)	2.5 ng/mL	1 vial
STANDARD E (0.5mL)	5.0 ng/mL	1 vial
STANDARD F (0.5mL)	10 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

CD63 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- CD63 antibody and an CD63-HRP conjugate. The assay sample and buffer are incubated together with CD63-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD63 concentration since CD63 from samples and CD63-HRP conjugate compete for the anti-CD63 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD63 from the sample, fewer sites are left to bind CD63-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD63 concentration in each sample is interpolated from this standard curve.

Quality Control on Rabbit Cluster of differentiation 63 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	89-114.79%
Linearity	Diluent Ratio	Range %
	1:2	96-116
	1:4	91-109
	1:8	88-103
Specificity/Cross-reactivity	No significant cross-reactivity or interference between CD63 and analogues was observed.