E04I0010 Rabbit Interleukin 1β ELISA kit
Rabbit Interleukin 1β ELISA kit is suitable for detecting samples from rabbit species. Interleukin 1β can also be called Catabolin, H1, IL-1β, Hematopoietin 1, IFN beta inducing factor, IL 1, IL 1 beta, IL 1B, IL1B, IL1F2, Interleukin 1 beta, Interleukin 1 beta precursor, LAF, OAF, Osteoclast activating factor, Preinterleukin beta, Pro interleukin 1 beta, IInterleukin-1 beta, IL 1β, IL1β, IL-1β.
Product Information | |
Cat. No. | E04I0010 |
Product Name | Rabbit Interleukin 1β ELISA kit |
Species | Rabbit |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IL 1β ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- IL 1β antibody and an IL 1β-HRP conjugate. The assay sample and buffer are incubated together with IL 1β-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL 1β concentration since IL 1β from samples and IL 1β-HRP conjugate compete for the anti-IL 1β antibody binding site. Since the number of sites is limited, as more sites are occupied by IL 1β from the sample, fewer sites are left to bind IL 1β-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 1β concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-114.79% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-116 | |
1:4 | 91-109 | |
1:8 | 88-103 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IL 1β and analogues was observed. | |
E04I0010 has been referenced in the below publications:
The Experimental Study of Perfluorocarbon Pretreatment Intratracheally in Rabbit Model of Acute Lung Injury.
The Experimental Study of Pretreatment Doses of Perfluorocarbon in Acute Lung Injury Rabbit.
The Effect of Total Flavonoids from Bergamot on Blood Lipid and Atherosclerosis Related Risk Fators in Hyperlipidemia Rabbits.
Role of NLRP3-Inflammasome/Caspase-1/Galectin-3 Pathway on Atrial Remodeling in Diabetic Rabbits.
N-acetylcysteine advancement of surfactant therapy in experimental meconium aspiration syndrome: possible mechanisms.
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