E04I0310 Rabbit Interleukin 18 ELISA kit
Rabbit Interleukin 18 ELISA kit is suitable for the detection of samples from Rabbit species. Interleukin 18 can also be called IGIF, interferon gamma-inducing factor IGIF, Interferon gamma-inducing factor, IFN-gamma-inducing factor, Interleukin-1 gamma, Iboctadekin, IFN gamma inducing factor, IL 1 gamma, IL 1g, IL1 gamma, IL18 protein, IL1F4, Interferon gamma inducing factor, IL18, IL 18, IL-18.
Product Information | |
Cat. No. | E04I0310 |
Product Name | Rabbit Interleukin 18 ELISA kit |
Species | Rabbit |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IL18 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IL18. Standards or samples are then added to the microtiter plate wells and IL18 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IL18 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IL18 are added to each well to “sandwich” the IL18 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL18 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL18 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 86-115% | |
Linearity | Diluent Ratio | Range % |
1:2 | 84-114 | |
1:4 | 87-117 | |
1:8 | 80-112 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IL18 and analogues was observed. | |
E04I0310 has been referenced in the below publications:
Role of NLRP3-Inflammasome/Caspase-1/Galectin-3 Pathway on Atrial Remodeling in Diabetic Rabbits.
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