Name: Rabbit Interleukin 6 ELISA kit (E04I0006)
Brand: Shanghai BlueGene Biotech CO., LTD.
Price: 100 USD

BlueGene Biotech Rabbit Interleukin 6 ELISA kit (E04I0006)

E04I0006 Rabbit Interleukin 6 ELISA kit

Rabbit Interleukin 6 ELISA kit is suitable for detecting samples from rabbit species. Interleukin 6 can also be called Interleukin BSF 2, B cell differentiation factor, B cell stimulatory factor 2, BSF2, CDF, CTL differentiation factor, Cytotoxic T cell differentiation factor, Hepatocyte stimulating factor, HGF, HPGF, HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL6 protein, Interferon beta 2, Interleukin 6 (interferon beta 2), IL 6, IL-6, IL6.

Products

Specifications of Rabbit Interleukin 6 ELISA kit

Product Information
Cat. No.	E04I0006
Product Name	Rabbit Interleukin 6 ELISA kit
Species	Rabbit
Product Size	48 Tests / 96 Tests
Concentration	50-1000 pg/mL
Sensitivity	1.0 pg/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/mL	1 vial
STANDARD B (0.5mL)	50 pg/mL	1 vial
STANDARD C (0.5mL)	100 pg/mL	1 vial
STANDARD D (0.5mL)	250 pg/mL	1 vial
STANDARD E (0.5mL)	500 pg/mL	1 vial
STANDARD F (0.5mL)	1000 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

IL 6 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- IL 6 antibody and an IL 6-HRP conjugate. The assay sample and buffer are incubated together with IL 6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL 6 concentration since IL 6 from samples and IL 6-HRP conjugate compete for the anti-IL 6 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL 6 from the sample, fewer sites are left to bind IL 6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 6 concentration in each sample is interpolated from this standard curve.

Quality Control on Rabbit Interleukin 6 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	89-114.79%
Linearity	Diluent Ratio	Range %
	1:2	96-116
	1:4	91-109
	1:8	88-103
Specificity/Cross-reactivity	No significant cross-reactivity or interference between IL 6 and analogues was observed.

BlueGene Biotech Product Show

Summary of the Assay Procedure for Rabbit Interleukin 6 ELISA kit

Citations of Rabbit Interleukin 6 ELISA kit

E04I0006 has been referenced in the below publications:

The effects and mechanism ofbone marrow mesenchymal stem cells transplantation on the stability of vulnerable plaque.

The impact of Ultrasound-guided cryoablation on the local scarring and inflammation and it's application in breast benign lesions.

Influence of exogenous putrescine and cadaverine on pro-inflammatory factors in the peripheral blood of rabbits.

The Study about Mechanism of Onion stalk extraction on Prevention and Treatment of Rabbits’Traumatic Deep Vein Thrombosis.

Effect of necrotic wound tissue decomposition products on serum inflammation factors in rabbits.

Transplantation of human umbilical cord mesenchymal stem cells in the treatment of systemic lupus erythematosus.

Comparison of two fluid solutions for resuscitation in a rabbit model of crush syndrome.

Long-term topical application of preservative-free prostaglandin analogues evokes macrophage infiltration in the ocular adnexa.