Name: Rabbit Receptor Activator of Nuclear Factor Kb Ligand ELISA kit (E04R0005)
Brand: Shanghai BlueGene Biotech CO., LTD.
Price: 100 USD

BlueGene Biotech Rabbit Receptor Activator of Nuclear Factor Kb Ligand ELISA kit (E04R0005)

Cardiovascular

Immunology

E04R0005 Rabbit Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

Rabbit Receptor Activator of Nuclear Factor Kb Ligand ELISA kit is suitable for detecting samples from rabbit species. Receptor Activator of Nuclear Factor Kb Ligand can also be called CD254, TNFSF11, ODF, OPGL, TRANCE, HRANKL2, SOdf, Tumor Necrosis Factor(ligand)superfamily Member 11, TNF-related activation-induced cytokine, RANKL.

Products

Rabbit Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

Product Information
Cat. No.	E04R0005
Product Name	Rabbit Receptor Activator of Nuclear Factor Kb Ligand ELISA kit
Species	Rabbit
Product Size	48 Tests / 96 Tests
Concentration	0.5-10ng/mL
Sensitivity	0.1 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	0.5 ng/mL	1 vial
STANDARD C (0.5mL)	1.0 ng/mL	1 vial
STANDARD D (0.5mL)	2.5 ng/mL	1 vial
STANDARD E (0.5mL)	5.0 ng/mL	1 vial
STANDARD F (0.5mL)	10 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

RANKL ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- RANKL antibody and an RANKL-HRP conjugate. The assay sample and buffer are incubated together with RANKL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RANKL concentration since RANKL from samples and RANKL-HRP conjugate compete for the anti-RANKL antibody binding site. Since the number of sites is limited, as more sites are occupied by RANKL from the sample, fewer sites are left to bind RANKL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RANKL concentration in each sample is interpolated from this standard curve.

Quality Control on Rabbit Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	89-114.79%
Linearity	Diluent Ratio	Range %
	1:2	96-116
	1:4	91-109
	1:8	88-103
Specificity/Cross-reactivity	No significant cross-reactivity or interference between RANKL and analogues was observed.

BlueGene Biotech Product Show

Summary of the Assay Procedure for Rabbit Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

Citations of Rabbit Receptor Activator of Nuclear Factor Kb Ligand ELISA kit

E04R0005 has been referenced in the below publications:

Effects of parathyroid hormone on OPG/RANKL/RANK signaling during distraction osteogenesis in a rabbit mandibular lengthening model.