E04T0008 Rabbit Tumor Necrosis Factor Alpha ELISA kit
Rabbit Tumor Necrosis Factor Alpha ELISA kit is suitable for detecting samples from rabbit species. Tumor Necrosis Factor Alpha can also be called DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2, TNFAlpha, TNF a, TNF-a, TNFa.
Product Information | |
Cat. No. | E04T0008 |
Product Name | Rabbit Tumor Necrosis Factor Alpha ELISA kit |
Species | Rabbit |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TNFa ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti- TNFa antibody and an TNFa-HRP conjugate. The assay sample and buffer are incubated together with TNFa-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TNFa concentration since TNFa from samples and TNFa-HRP conjugate compete for the anti-TNFa antibody binding site. Since the number of sites is limited, as more sites are occupied by TNFa from the sample, fewer sites are left to bind TNFa-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TNFa concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-114.79% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-116 | |
1:4 | 91-109 | |
1:8 | 88-103 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TNFa and analogues was observed. | |
E04T0008 has been referenced in the below publications:
TIMP‑3 suppresses the proliferation and migration of SMCs from the aortic neck of atherosclerotic AAA in rabbits, via decreased MMP‑2 and MMP‑9 activity, and reduced TNF‑α expression.
The effects and mechanism ofbone marrow mesenchymal stem cells transplantation on the stability of vulnerable plaque.
The effets of microwave on osteoarthritis in knee of rabbits.
The impact of Ultrasound-guided cryoablation on the local scarring and inflammation and it's application in breast benign lesions.
Effect of ischemic postconditioning on NF-kB activation signaling pathway in early lung transplantation.
The Experimental Study of Perfluorocarbon Pretreatment Intratracheally in Rabbit Model of Acute Lung Injury.
Effect of necrotic wound tissue decomposition products on serum inflammation factors in rabbits.
Effects of xilei oral sustained-release mucoadhesive films on expression of TNF- a, IL-1 in RAU rabbits models.
The Experimental Study of Pretreatment Doses of Perfluorocarbon in Acute Lung Injury Rabbit.
Transplantation of human umbilical cord mesenchymal stem cells in the treatment of systemic lupus erythematosus.
N-acetylcysteine advancement of surfactant therapy in experimental meconium aspiration syndrome: possible mechanisms.
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