E02B0004 Rat B7 Homolog 3 ELISA kit
The Rat B7 Homolog 3 ELISA kit can be used to identify samples from the rat species. B7 Homolog 3 can also be called CD276, 4Ig-B7-H3, B7-H3, B7H3, B7RP-2, CD276 molecule.
Specifications of Rat B7 Homolog 3 ELISA kit
Product Information | |
Cat. No. | E02B0004 |
Product Name | Rat B7 Homolog 3 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
B7-H3 ELISA kit uses an anti-B7-H3 antibody and an B7-H3-HRP conjugate in a competitive enzyme immunoassay method. B7-H3-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-B7-H3 antibody binding site between B7-H3 from samples and B7-H3-HRP conjugate, the intensity of the color is inversely proportional to the concentration of B7-H3. Since the number of sites is limited, as more sites are occupied by B7-H3 from the sample, fewer sites are left to bind B7-H3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The B7-H3 concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat B7 Homolog 3 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between B7-H3 and analogues was observed. | |
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Summary of the Assay Procedure for Rat B7 Homolog 3 ELISA kit
Citations of Rat B7 Homolog 3 ELISA kit
E02B0004 has been referenced in the below publications：
Neutrophil extracellular traps enhance procoagulant activity in patients with oral squamous cell carcinoma.
