E02C0009 Rat C Reactive Protein ELISA kit
The Rat C Reactive Protein ELISA kit can be used to identify samples from the rat species. C Reactive Protein can also be called PTX 1, PTX1, C-reactive protein, pentraxin-related, C-Reactive Protein, CRP.
Specifications of Rat C Reactive Protein ELISA kit
Product Information | |
Cat. No. | E02C0009 |
Product Name | Rat C Reactive Protein ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 5-100 ug/mL |
Sensitivity | 1.0 ug/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/ml | 1 vial |
STANDARD B (0.5mL) | 5.0 ug/ml | 1 vial |
STANDARD C (0.5mL) | 10 ug/ml | 1 vial |
STANDARD D (0.5mL) | 25 ug/ml | 1 vial |
STANDARD E (0.5mL) | 50 ug/ml | 1 vial |
STANDARD F (0.5mL) | 100 ug/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CRP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CRP. Standards or samples are then added to the microtiter plate wells and CRP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CRP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CRP are added to each well to “sandwich” the CRP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CRP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CRP concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat C Reactive Protein ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CRP and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat C Reactive Protein ELISA kit
Citations of Rat C Reactive Protein ELISA kit
E02C0009 has been referenced in the below publications：
Effect of dietary fiber on intestinal mucosa barrie in rats with experimental colitis—TNF-a, CRP concentration and morphological changes of small intestines.
The Effects of Astraglus Polysaccharides on Oxidative Stress and Inflammatory Responses in Severely Burned Rats.
种中药组合物在制备治疗细颗粒造成肺损伤药物中的应用。
Effect of dietary fiber on intestinal mucosa barrie in rats with experimental colitis.
