E02S0067 Rat Chemokine Ligand 12 ELISA Kit
The Rat Chemokine Ligand 12 ELISA Kit can be used to identify samples from the rat species. Chemokine Ligand 12 can also be called SDF1 alpha, C-X-C motif chemokine 12, Chemokine (C X C motif) ligand 12, CXCL12, hIRH, hSDF-1, Intercrine reduced in hepatomas, IRH, PBSF, PBSF, Pre B cell growth stimulating factor, Pre-B cell growth-stimulating factor, SCYB12, SDF 1 alpha, SDF-1, SDF 1 beta, SDF 1b, SDF-1, SDF-1-alpha(3-67), SDF1.
Product Information | |
Cat. No. | E02S0067 |
Product Name | Rat Chemokine Ligand 12 ELISA Kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000 pg/mL |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 250 pg/mL | 1 vial |
STANDARD C (0.5mL) | 500 pg/mL | 1 vial |
STANDARD D (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD E (0.5mL) | 2500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 5000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CXCL12 ELISA kit uses an anti-CXCL12 antibody and an CXCL12-HRP conjugate in a competitive enzyme immunoassay method. CXCL12-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CXCL12 antibody binding site between CXCL12 from samples and CXCL12-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CXCL12. Since the number of sites is limited, as more sites are occupied by CXCL12 from the sample, fewer sites are left to bind CXCL12-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CXCL12 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CXCL12 and analogues was observed. | |
E02S0067 has been referenced in the below publications：
Anti-Thymocyte Globulin Induces Neoangiogenesis and Preserves Cardiac Function after Experimental Myocardial Infarction.
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