E02C0563 Rat Connective Tissue Growth Factor ELISA kit
The Rat Connective Tissue Growth Factor ELISA kit can be used to identify samples from the rat species. Connective Tissue Growth Factor can also be called CTGF, CCN2, NOV2, HCS24, IGFBP8.
Specifications of Rat Connective Tissue Growth Factor ELISA kit
Product Information | |
Cat. No. | E02C0563 |
Product Name | Rat Connective Tissue Growth Factor ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000 pg/mL |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 250 pg/mL | 1 vial |
STANDARD C (0.5mL) | 500 pg/mL | 1 vial |
STANDARD D (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD E (0.5mL) | 2500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 5000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CTGF ELISA kit uses an anti-CTGF antibody and an CTGF-HRP conjugate in a competitive enzyme immunoassay method. CTGF-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CTGF antibody binding site between CTGF from samples and CTGF-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CTGF. Since the number of sites is limited, as more sites are occupied by CTGF from the sample, fewer sites are left to bind CTGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTGF concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Connective Tissue Growth Factor ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CTGF and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Connective Tissue Growth Factor ELISA kit
Citations of Rat Connective Tissue Growth Factor ELISA kit
E02C0563 has been referenced in the below publications：
祖师麻肠溶制剂的药效学研究。
