E02E0166 Rat Epoxyeicosatrienoic acids ELISA kit
The Rat Epoxyeicosatrienoic acids ELISA kit can be used to identify samples from the rat species. Epoxyeicosatrienoic acids can also be called EETs.
Specifications of Rat Epoxyeicosatrienoic acids ELISA kit
Product Information | |
Cat. No. | E02E0166 |
Product Name | Rat Epoxyeicosatrienoic acids ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
EETs ELISA kit uses an anti-EETs antibody and an EETs-HRP conjugate in a competitive enzyme immunoassay method. EETs-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-EETs antibody binding site between EETs from samples and EETs-HRP conjugate, the intensity of the color is inversely proportional to the concentration of EETs. Since the number of sites is limited, as more sites are occupied by EETs from the sample, fewer sites are left to bind EETs-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EETs concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Epoxyeicosatrienoic acids ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between EETs and analogues was observed. | |
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Summary of the Assay Procedure for Rat Epoxyeicosatrienoic acids ELISA kit
Citations of Rat Epoxyeicosatrienoic acids ELISA kit
E02E0166 has been referenced in the below publications：
Inhibition of Epoxyeicosatrienoic Acid Production in Rats With Cirrhosis Has Beneficial Effects on Portal Hypertension by Reducing Splanchnic Vasodilation.
