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BlueGene Biotech Rat Granulocyte Macrophage Colony Stimulating Factor ELISA kit (E02G0016)

E02G0016 Rat Granulocyte Macrophage Colony Stimulating Factor ELISA kit

The Rat Granulocyte Macrophage Colony Stimulating Factor ELISA kit can be used to identify samples from the rat species. Granulocyte Macrophage Colony Stimulating Factor can also be called CSF2, GMCSF,  colony stimulating factor 2, CSF, GM CSF.

Products

Specifications of Rat Granulocyte Macrophage Colony Stimulating Factor ELISA kit

Product Information

Cat. No.

E02G0016

Product Name

Rat Granulocyte Macrophage Colony Stimulating Factor ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

GM CSF ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GM CSF. Standards or samples are then added to the microtiter plate wells and GM CSF if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GM CSF present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GM CSF are added to each well to “sandwich” the GM CSF immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GM CSF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GM CSF concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Granulocyte Macrophage Colony Stimulating Factor ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between GM CSF and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Granulocyte Macrophage Colony Stimulating Factor ELISA kit

Summary of the Assay Procedure for Rat Granulocyte Macrophage Colony Stimulating Factor ELISA kit

Citations of Rat Granulocyte Macrophage Colony Stimulating Factor ELISA kit

E02G0016 has been referenced in the below publications:

Effects of colon targeting microecological modulator nano CYP synbiotics on cytokines and expression of SOD,MDA, NO and MPO in dysbacteria wistar rats.


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