E02C0087 Rat High sensitivity C Reactive Protein ELISA kit
The Rat High sensitivity C Reactive Protein ELISA kit can be used to identify samples from the rat species. High sensitivity C Reactive Protein can also be called CRP, PTX1, C-RP, Pentraxin-Related, Pentraxin 1, C-reactive protein, pentraxin-related, C-Reactive Protein, Hs CRP.
Product Information | |
Cat. No. | E02C0087 |
Product Name | Rat High sensitivity C Reactive Protein ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 5-100 ng/mL |
Sensitivity | 1.0 ng/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/ml | 1 vial |
STANDARD C (0.5mL) | 10 ng/ml | 1 vial |
STANDARD D (0.5mL) | 25 ng/ml | 1 vial |
STANDARD E (0.5mL) | 50 ng/ml | 1 vial |
STANDARD F (0.5mL) | 100 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Hs CRP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Hs CRP. Standards or samples are then added to the microtiter plate wells and Hs CRP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of Hs CRP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for Hs CRP are added to each well to “sandwich” the Hs CRP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Hs CRP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Hs CRP concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Hs CRP and analogues was observed. | |
E02C0087 has been referenced in the below publications：
Effects of exercise traning on ventricular remodeling following myocardial infarction in rats.
Effects of periodontal mechanical therapy with local and systemic drugs on carotid artery and serum high-sensitivity C-reactive protein in rats with chronic periodontitis associated with atherosclerosis.
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