E02I0037 Rat Immunoglobulin E ELISA kit
The Rat Immunoglobulin E ELISA kit can be used to identify samples from the rat species. Immunoglobulin E can also be called IGHE, Immunoglobulin Heavy Constant Epsilon, Ig epsilon chain C region, IgE.
Specifications of Rat Immunoglobulin E ELISA kit
Product Information | |
Cat. No. | E02I0037 |
Product Name | Rat Immunoglobulin E ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 mg/l |
Sensitivity | 1.0 mg/l |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 mg/l | 1 vial |
STANDARD B (0.5mL) | 50 mg/l | 1 vial |
STANDARD C (0.5mL) | 100 mg/l | 1 vial |
STANDARD D (0.5mL) | 250 mg/l | 1 vial |
STANDARD E (0.5mL) | 500 mg/l | 1 vial |
STANDARD F (0.5mL) | 1000 mg/l | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IgE ELISA kit uses an anti-IIgE antibody and an IIgE-HRP conjugate in a competitive enzyme immunoassay method. IIgE-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IIgE antibody binding site between IIgE from samples and IIgE-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IIgE. Since the number of sites is limited, as more sites are occupied by IIgE from the sample, fewer sites are left to bind IIgE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IIgE concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Immunoglobulin E ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IgE and analogues was observed. | |
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Summary of the Assay Procedure for Rat Immunoglobulin E ELISA kit
Citations of Rat Immunoglobulin E ELISA kit
E02I0037 has been referenced in the below publications：
Changes of Serum IgE and Tryptase in Anaphylactic Shock Rats.
Mechanisms of action of Shizhenqing granules for eczema treatment: Network pharmacology analysis and experimental validation.
