E02I0058 Rat Immunoglobulin G ELISA kit
The Rat Immunoglobulin G ELISA kit can be used to identify samples from the rat species. Immunoglobulin G can also be called IgG.
Specifications of Rat Immunoglobulin G ELISA kit
Product Information | |
Cat. No. | E02I0058 |
Product Name | Rat Immunoglobulin G ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 mg/ml |
Sensitivity | 0.1 mg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 mg/ml | 1 vial |
STANDARD B (0.5mL) | 2.5 mg/ml | 1 vial |
STANDARD C (0.5mL) | 5.0 mg/ml | 1 vial |
STANDARD D (0.5mL) | 10 mg/ml | 1 vial |
STANDARD E (0.5mL) | 25 mg/ml | 1 vial |
STANDARD F (0.5mL) | 50 mg/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IgG ELISA kit uses an anti-IIgG antibody and an IIgG-HRP conjugate in a competitive enzyme immunoassay method. IIgG-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IIgG antibody binding site between IIgG from samples and IIgG-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IIgG. Since the number of sites is limited, as more sites are occupied by IIgG from the sample, fewer sites are left to bind IIgG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IIgG concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Immunoglobulin G ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IgG and analogues was observed. | |
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Summary of the Assay Procedure for Rat Immunoglobulin G ELISA kit
Citations of Rat Immunoglobulin G ELISA kit
E02I0058 has been referenced in the below publications：
The research on the relation between the internal environment of prostate of rats with chronic prostatitis and kidney deficiency.
祖师麻肠溶制剂的药效学研究。
