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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Immunoglobulin G ELISA kit (E02I0058)

E02I0058 Rat Immunoglobulin G ELISA kit

The Rat Immunoglobulin G ELISA kit can be used to identify samples from the rat species. Immunoglobulin G can also be called IgG.

Products

Specifications of Rat Immunoglobulin G ELISA kit

Product Information

Cat. No.

E02I0058

Product Name

Rat Immunoglobulin G ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 mg/ml

Sensitivity

0.1 mg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 mg/ml

1 vial

STANDARD B (0.5mL)

2.5 mg/ml

1 vial

STANDARD C (0.5mL)

5.0 mg/ml

1 vial

STANDARD D (0.5mL)

10 mg/ml

1 vial

STANDARD E (0.5mL)

25 mg/ml

1 vial

STANDARD F (0.5mL)

50 mg/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IgG ELISA kit uses an anti-IIgG antibody and an IIgG-HRP conjugate in a competitive enzyme immunoassay method. IIgG-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IIgG antibody binding site between IIgG from samples and IIgG-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IIgG. Since the number of sites is limited, as more sites are occupied by IIgG from the sample, fewer sites are left to bind IIgG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IIgG concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Immunoglobulin G ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IgG and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Immunoglobulin G ELISA kit

Summary of the Assay Procedure for Rat Immunoglobulin G ELISA kit

Citations of Rat Immunoglobulin G ELISA kit

E02I0058 has been referenced in the below publications:

The research on the relation between the internal environment of prostate of rats with chronic prostatitis and kidney deficiency.

祖师麻肠溶制剂的药效学研究。

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