E02I0310 Rat Interleukin 18 ELISA kit
The Rat Interleukin 18 ELISA kitcan be used to identify samples from the rat species. Interleukin 18 can also be called IGIF, interferon gamma-inducing factor IGIF, Interferon gamma-inducing factor, IFN-gamma-inducing factor, Interleukin-1 gamma, Iboctadekin, IFN gamma inducing factor, IL 1 gamma, IL 1g, IL1 gamma, IL18 protein, IL1F4, Interferon gamma inducing factor, IL18, IL-18, IL 18.
Product Information | |
Cat. No. | E02I0310 |
Product Name | Rat Interleukin 18 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IL18 ELISA kit uses an anti-IL18 antibody and an IL18-HRP conjugate in a competitive enzyme immunoassay method. IL18-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IL18 antibody binding site between IL18 from samples and IL18-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IL18. Since the number of sites is limited, as more sites are occupied by IL18 from the sample, fewer sites are left to bind IL18-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL18 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IL18 and analogues was observed. | |
E02I0310 has been referenced in the below publications：
Study on the anti-gout effect and mechanism of baicalin.
NLRP3 Inflammasome Is Involved in Calcium-Sensing Receptor-Induced Aortic Remodeling in SHRs.
NLRP3 Inflammasome is involved in Calcium-Sensing Receptor-induced aortic remodeling in SHRs.
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