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BlueGene Biotech Rat Interleukin 33 ELISA kit (E02I0204)

E02I0204 Rat Interleukin 33 ELISA kit

The Rat Interleukin 33 ELISA kit can be used to identify samples from the rat species. Interleukin 33 can also be called DV27, C9ORF26, IL1F11, NFHEV, Interleukin-1 Family, Member 11, Nuclear factor from high endothelial venules, IL33, IL-33, IL 33.

Products

Specifications of Rat Interleukin 33 ELISA kit

Product Information

Cat. No.

E02I0204

Product Name

Rat Interleukin 33 ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IL 33 ELISA kit uses an anti-IL 33 antibody and an IL 33-HRP conjugate in a competitive enzyme immunoassay method. IL 33-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IL 33 antibody binding site between IL 33 from samples and IL 33-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IL 33. Since the number of sites is limited, as more sites are occupied by IL 33 from the sample, fewer sites are left to bind IL 33-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 33 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Interleukin 33 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IL 33 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Interleukin 33 ELISA kit

Summary of the Assay Procedure for Rat Interleukin 33 ELISA kit

Citations of Rat Interleukin 33 ELISA kit

E02I0204 has been referenced in the below publications:

Effects of exercise traning on ventricular remodeling following myocardial infarction in rats.


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