E02K0025 Rat Kidney Injury Molecule 1 ELISA
The Rat Kidney Injury Molecule 1 ELISA can be used to identify samples from the rat species. Kidney Injury Molecule 1 can also be called HAVCR1, TIM1, TIMD1, HAVCR, Hepatitis A Virus Cellular Receptor 1, T Cell Immunoglobulin And Mucin Domain-Containing Protein 1, T-cell immunoglobulin mucin receptor 1.
Product Information | |
Cat. No. | E02K0025 |
Product Name | Rat Kidney Injury Molecule 1 ELISA |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000 pg/mL |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 250 pg/mL | 1 vial |
STANDARD C (0.5mL) | 500 pg/mL | 1 vial |
STANDARD D (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD E (0.5mL) | 2500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 5000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
KIM1 ELISA kit uses an anti-KIM1 antibody and an KIM1-HRP conjugate in a competitive enzyme immunoassay method. KIM1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-KIM1 antibody binding site between KIM1 from samples and KIM1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of KIM1. Since the number of sites is limited, as more sites are occupied by KIM1 from the sample, fewer sites are left to bind KIM1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KIM1 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between KIM1 and analogues was observed. | |
E02K0025 has been referenced in the below publications：
Changes of NGAL and KIM-1 in the early period of acute kidney injury by lipopolysaccharide-induced in rats.
Effects of Compound Sevenlobed Yam Rhizome Soup on NGAL and KIM-1 in Urine of Uric Acid Nephropathy Rats.
Protective effects of sinomenine on acute renal injury in septic rats.
Protective Effect of Shenkang Injections in Rats with K idney Injury induced by Gentamicin.
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