En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Kidney Injury Molecule 1 ELISA (E02K0025)

E02K0025 Rat Kidney Injury Molecule 1 ELISA

The Rat Kidney Injury Molecule 1 ELISA can be used to identify samples from the rat species. Kidney Injury Molecule 1 can also be called HAVCR1, TIM1, TIMD1, HAVCR, Hepatitis A Virus Cellular Receptor 1, T Cell Immunoglobulin And Mucin Domain-Containing Protein 1, T-cell immunoglobulin mucin receptor 1.

Products

Specifications of Rat Kidney Injury Molecule 1 ELISA

Product Information

Cat. No.

E02K0025

Product Name

Rat Kidney Injury Molecule 1 ELISA

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

250-5000 pg/mL

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

250 pg/mL

1 vial

STANDARD C (0.5mL)

500 pg/mL

1 vial

STANDARD D (0.5mL)

1000 pg/mL

1 vial

STANDARD E (0.5mL)

2500 pg/mL

1 vial

STANDARD F (0.5mL)

5000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

KIM1 ELISA kit uses an anti-KIM1 antibody and an KIM1-HRP conjugate in a competitive enzyme immunoassay method. KIM1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-KIM1 antibody binding site between KIM1 from samples and KIM1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of KIM1. Since the number of sites is limited, as more sites are occupied by KIM1 from the sample, fewer sites are left to bind KIM1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KIM1 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Kidney Injury Molecule 1 ELISA

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between KIM1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Kidney Injury Molecule 1 ELISA

Summary of the Assay Procedure for Rat Kidney Injury Molecule 1 ELISA

Citations of Rat Kidney Injury Molecule 1 ELISA

E02K0025 has been referenced in the below publications:

Changes of NGAL and KIM-1 in the early period of acute kidney injury by lipopolysaccharide-induced in rats.

Effects of Compound Sevenlobed Yam Rhizome Soup on NGAL and KIM-1 in Urine of Uric Acid Nephropathy Rats.

Protective effects of sinomenine on acute renal injury in septic rats.

Protective Effect of Shenkang Injections in Rats with K idney Injury induced by Gentamicin.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.