E02L0205 Rat Lipoprotein associated Phospholipase A2 ELISA kit
The Rat Lipoprotein associated Phospholipase A2 ELISA kit can be used to identify samples from the rat species. Lipoprotein associated Phospholipase A2 can also be called Lp PL A2.
Specifications of Rat Lipoprotein associated Phospholipase A2 ELISA kit
Product Information | |
Cat. No. | E02L0205 |
Product Name | Rat Lipoprotein associated Phospholipase A2 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Lp PL A2 ELISA kit uses an anti-Lp PL A2 antibody and an Lp PL A2-HRP conjugate in a competitive enzyme immunoassay method. Lp PL A2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Lp PL A2 antibody binding site between Lp PL A2 from samples and Lp PL A2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Lp PL A2. Since the number of sites is limited, as more sites are occupied by Lp PL A2 from the sample, fewer sites are left to bind Lp PL A2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Lp PL A2 concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Lipoprotein associated Phospholipase A2 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Lp PL A2 and analogues was observed. | |
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Summary of the Assay Procedure for Rat Lipoprotein associated Phospholipase A2 ELISA kit
Citations of Rat Lipoprotein associated Phospholipase A2 ELISA kit
E02L0205 has been referenced in the below publications：
The expressions of lp-PLA2 and GPR4 in vessel endothelial dysfunction rats induced by fatigue stress and the effect of Tongluo intervention.
